| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
26/11/2008 |
|
Data da última atualização: |
01/12/2008 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
MORAIS-LINO, L. S.; SANTOS-SEREJO, J. A. dos; SILVA, S. de O. e; SANTANA, J. R. F. de; KOBAYASHI, A. K. |
|
Afiliação: |
Lucymeire Souza Morais-Lino, UEFS; Janay Almeida dos Santos-Serejo, CNPMF; Sebastião de Oliveira e Silva, CNPMF; José Raniere Ferreira de Santana, UEFS; Adilson Kenji Kobayashi, CPAMN. |
|
Título: |
Cell suspension culture and plant regeneration of a brazilian plantain, cultivar Terra. |
|
Ano de publicação: |
2008 |
|
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 10, p. 1325-1330, out. 2008. |
|
ISSN: |
0100-204X |
|
Idioma: |
Português |
|
Conteúdo: |
The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerate plants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 µM of IAA and 2.2 µM of BAP. |
|
Palavras-Chave: |
Propagation. |
|
Thesaurus Nal: |
culture media; Musa; somatic embryogenesis. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01669naa a2200229 a 4500
001 1655086
005 2008-12-01
008 2008 bl uuuu u00u1 u #d
022 $a0100-204X
100 1 $aMORAIS-LINO, L. S.
245 $aCell suspension culture and plant regeneration of a brazilian plantain, cultivar Terra.
260 $c2008
520 $aThe objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerate plants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 µM of IAA and 2.2 µM of BAP.
650 $aculture media
650 $aMusa
650 $asomatic embryogenesis
653 $aPropagation
700 1 $aSANTOS-SEREJO, J. A. dos
700 1 $aSILVA, S. de O. e
700 1 $aSANTANA, J. R. F. de
700 1 $aKOBAYASHI, A. K.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 43, n. 10, p. 1325-1330, out. 2008.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Nenhum exemplar cadastrado para este documento. |
Voltar
|
|
|
| Registros recuperados : 4 | |
| Registros recuperados : 4 | |
|
| Expressão de busca inválida. Verifique!!! |
|
|
